ArsC

• Description: arsenate reductase
  
  

Gene name	arsC
Synonyms	yqcM
Essential	no
Product	arsenate reductase
Function	detoxification of arsenate
Gene expression levels in SubtiExpress: arsC
Interactions involving this protein in SubtInteract: ArsC
MW, pI	15 kDa, 4.749
Gene length, protein length	417 bp, 139 aa
Immediate neighbours	spoIVCA, arsB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against toxic metals, Skin element

This gene is a member of the following regulons

ArsR regulon, SigF regulon

The gene

Basic information

• Locus tag: BSU25780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot)

• Protein family: ArsC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ArsC-TrxA PubMed

• Localization:

Database entries

• Structure: 1Z2D (reduced form), 1Z2E (oxidized form), 2IPA (TrxA-ArsC-complex) PubMed, 1JL3

• UniProt: P45947

• KEGG entry: [3]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon: arsR-yqcK-arsB-arsC PubMed

• Expression browser: arsC PubMed

• Sigma factor: SigA PubMed, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • expressed in the presence of toxic metal ions (As(III), Cd(II) and Ag(I)) (ArsR) PubMed

• Regulatory mechanism:
  • ArsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

You Li, Yunfei Hu, Xinxin Zhang, Huimin Xu, Ewen Lescop, Bin Xia, Changwen Jin
Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis.
J Biol Chem: 2007, 282(15);11078-83
[PubMed:17303556] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Z Guan, L Hederstedt, J Li, X D Su
Preparation and crystallization of a Bacillus subtilis arsenate reductase.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 11);1718-21
[PubMed:11679756] [WorldCat.org] [DOI] (P p)

T Sato, Y Kobayashi
The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.
J Bacteriol: 1998, 180(7);1655-61
[PubMed:9537360] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed