ComEB

• Description: late competence protein required for DNA binding and uptake
  
  

Gene name	comEB
Synonyms	comD
Essential	no
Product	late competence protein
Function	genetic compentence
Gene expression levels in SubtiExpress: comEB
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Protein secretion
MW, pI	20 kDa, 5.368
Gene length, protein length	567 bp, 189 aa
Immediate neighbours	comEC, comEA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon, ComN regulon

The gene

Basic information

• Locus tag: BSU25580

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • localizes to one or both cell poles PubMed

Database entries

• Structure:

• UniProt: P32393

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comEA-comEB-comEC PubMed

• Expression browser: comEB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • post-translational control by ComN PubMed
  • yutB is required for transcription PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523] [WorldCat.org] [DOI] (P p)