Fur

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  • Description: transcription regulator of iron homoeostasis

Gene name fur
Synonyms yqkL
Essential no
Product transcriptional repressor Fur family
Function regulation of iron homoeostasis

and transcription of ferri-siderophore uptake genes

Gene expression levels in SubtiExpress: fur

and transcription of ferri-siderophore uptake genes

Metabolic function and regulation of this protein in SubtiPathways:
Metal ion homeostasis, Alternative nitrogen sources, Protein secretion
MW, pI 17 kDa, 5.374
Gene length, protein length 447 bp, 149 aa
Immediate neighbours yqzK, spoIIM
Sequences Protein DNA DNA_with_flanks
Genetic context
Fur context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Fur expression.png



















Categories containing this gene/protein

iron metabolism, transcription factors and their control

This gene is a member of the following regulons

PerR regulon

The Fur regulon

The gene

Basic information

  • Locus tag: BSU23520

Phenotypes of a mutant

  • no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to FsrA-mediated repression of the gltA-gltB operon) PubMed
  • poor frowth on lactate as single carbon source (due to overexpression of FsrA-mediated repression of the lutA-lutB-lutC operon, can be suppressed by inactivation of fsrA or fbpB) PubMed
  • transcription profile of a fur mutant strain: GEO PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:
    • DNA binding activity (repression) is triggered by binding of Fe(II) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the absence of hydrogen peroxide (PerR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: HB6543 (aphA3), available in the John Helmann lab; also available in the Stülke lab GP879 (fur::mls) and GP868 (fur::mls, perR::spc)
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Reviews

Charles M Moore, John D Helmann
Metal ion homeostasis in Bacillus subtilis.
Curr Opin Microbiol: 2005, 8(2);188-95
[PubMed:15802251] [WorldCat.org] [DOI] (P p)

The Fur regulon

Addititonal publications: PubMed

Other original publications

Addititonal publications: PubMed


PubMed