XylB

• Description: xylulokinase
  
  

Gene name	xylB
Synonyms	yncA
Essential	no
Product	xylulokinase
Function	utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	55 kDa, 6.459
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	xylA, yncB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

• Locus tag: BSU17610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2NLX (from E. coli, 35% identity, 52% similarity) PubMed

• UniProt: P39211

• KEGG entry: [3]

• E.C. number: 2.7.1.17

Expression and regulation

• Operon: xylA-xylB PubMed

• Expression browser: xylB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • XylR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1151 (del aphA3) available in Stülke lab
  • 1A719 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage