MtlA

• Description: trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS
  
  

Gene name	mtlA
Synonyms
Essential	no
Product	trigger enzyme: mannitol-specific phosphotransferase system, EIICB
Function	mannitol uptake and phosphorylation, control of MtlR activity
Gene expression levels in SubtiExpress: mtlA
Interactions involving this protein in SubtInteract: MtlA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 8.814
Gene length, protein length	1434 bp, 478 aa
Immediate neighbours	ycnL, mtlF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

• Locus tag: BSU03981

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-559 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MtlA-MtlR PubMed (the non-phosphorylated IIB domain of MtlA interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of MtlR) PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1A3A (from Escherichia coli k12 mutant, 42% identity, 66% similarity) PubMed

• UniProt: P42956

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Expression browser: mtlA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Philippe Joyet, Houda Bouraoui, Francine Moussan Désirée Aké, Meriem Derkaoui, Arthur Constant Zébré, Thanh Nguyen Cao, Magali Ventroux, Sylvie Nessler, Marie-Françoise Noirot-Gros, Josef Deutscher, Eliane Milohanic
Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate: sugar phosphotransferase system.
Biochim Biophys Acta: 2013, 1834(7);1415-24
[PubMed:23318733] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed

Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)