FeuA

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  • Description: ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with YusV as ATPase

Gene name feuA
Synonyms
Essential no
Product ABC transporter for the siderophores Fe-enterobactin
and Fe-bacillibactin (binding protein)
Function acquisition of iron
Gene expression levels in SubtiExpress: feuA
Interactions involving this protein in SubtInteract: FeuA
Metabolic function and regulation of this protein in SubtiPathways:
Stress, Metal ion homeostasis
MW, pI 34 kDa, 8.018
Gene length, protein length 951 bp, 317 aa
Immediate neighbours feuB, btr
Sequences Protein DNA DNA_with_flanks
Genetic context
FeuA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FeuA expression.png




























Categories containing this gene/protein

ABC transporters, acquisition of iron, iron metabolism, membrane proteins

This gene is a member of the following regulons

Btr regulon, CitB regulon, Fur regulon

The gene

Basic information

  • Locus tag: BSU01630

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/biological activity:

Substrate binding protein for the triscatecholate siderophores ferri-bacillibactin and ferri-enterobactin and part of the FeuA-FeuB-FeuC-YusV ATP-binding cassette-type transporter. Furthermore ferric complexes of L-norepinephrine are bound by this protein.

  • Protein family: Fe/B12 periplasmic-binding domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:

Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

  • Modification:
    • FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2PHZ, 2WI8, 2WHY (complex with ferri-bacillibactin), 2XUZ (complex with ferri-enterobactin), 2XV1 (complex with ferric mecam)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of iron (Fur, CitB) PubMed
    • induced in the presence of iron chelators bacillibactin or enterobactin (Btr) PubMed
  • Regulatory mechanism:
    • Fur: transcription repression PubMed
    • CitB: binding to the iron responsive element in the absence of iron
    • Btr: transcription activation in the presence of the co-activators bacillibactin or enterobactin PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed