PdaB

• Description: polysaccharide deacetylase
  
  

Gene name	pdaB
Synonyms	ybaN
Essential	no
Product	polysaccharide deacetylase
Function	spore cortex formation
Gene expression levels in SubtiExpress: pdaB
MW, pI	28 kDa, 9.182
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	kbaA, rrnI-16S
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU01570

Phenotypes of a mutant

pdaB ytrI and pdaB ytrH mutants are deficient in sporulation

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P50865

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pdaB PubMed

• Expression browser: pdaB PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation in the mother cell PubMed

• Regulatory mechanism: SpoIIID: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsuya Fukushima, Taichi Tanabe, Hiroki Yamamoto, Shigeo Hosoya, Tsutomu Sato, Hirofumi Yoshikawa, Junichi Sekiguchi
Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
J Biochem: 2004, 136(3);283-91
[PubMed:15598884] [WorldCat.org] [DOI] (P p)

Jessica M Silvaggi, David L Popham, Adam Driks, Patrick Eichenberger, Richard Losick
Unmasking novel sporulation genes in Bacillus subtilis.
J Bacteriol: 2004, 186(23);8089-95
[PubMed:15547282] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)