Ctc

• Description: general stress protein, similar to ribosomal protein L25
  
  

Gene name	ctc
Synonyms
Essential	no
Product	ribosomal protein
Function	unknown
Gene expression levels in SubtiExpress: ctc
Interactions involving this protein in SubtInteract: Ctc
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Murein recycling
MW, pI	21 kDa, 4.216
Gene length, protein length	612 bp, 204 aa
Immediate neighbours	prs, spoVC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU00520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Ctc subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • 5S rRNA-Ctc PubMed

• Localization: in the ribosome (large subunit) PubMed

Database entries

• Structure: 1FEU (from Thermus thermophilus, 32% identity, 53% similarity) PubMed

• UniProt: P14194

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:
  • gcaD-prs-ctc PubMed
  • ctc PubMed

• Expression browser: ctc PubMed

• Sigma factor:
  • gcaD: SigA PubMed
  • ctc: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism: alternative sigma factor, SigB PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP500(spc), available in Stülke lab

• Expression vector:
  • expression/ purification with N-terminal His-tag in E. coli: pGP804 (in pWH844), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Stülke lab

References

Reviews

Additional reviews: PubMed

Original publications

Additional publications: PubMed

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Matthias Schmalisch, Ines Langbein, Jörg Stülke
The general stress protein Ctc of Bacillus subtilis is a ribosomal protein.
J Mol Microbiol Biotechnol: 2002, 4(5);495-501
[PubMed:12432960] [WorldCat.org] (P p)

R Fedorov, V Meshcheryakov, G Gongadze, N Fomenkova, N Nevskaya, M Selmer, M Laurberg, O Kristensen, S Al-Karadaghi, A Liljas, M Garber, S Nikonov
Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 7);968-76
[PubMed:11418764] [WorldCat.org] [DOI] (P p)

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J Bacteriol: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

C L Truitt, E A Weaver, W G Haldenwang
Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32).
Mol Gen Genet: 1988, 212(1);166-71
[PubMed:2836704] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J Bacteriol: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J Mol Biol: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

J F Ollington, W G Haldenwang, T V Huynh, R Losick
Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.
J Bacteriol: 1981, 147(2);432-42
[PubMed:6790515] [WorldCat.org] [DOI] (P p)

W G Haldenwang, R Losick
A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis.
Nature: 1979, 282(5736);256-60
[PubMed:116131] [WorldCat.org] [DOI] (P p)