HisB

• Description: imidazoleglycerol-phosphate dehydratase
  
  

Gene name	hisB
Synonyms
Essential	no
Product	imidazoleglycerol-phosphate dehydratase
Function	biosynthesis of histidine
Gene expression levels in SubtiExpress: hisB
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	21 kDa, 5.598
Gene length, protein length	582 bp, 194 aa
Immediate neighbours	hisH, hisD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU34900

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O (according to Swiss-Prot)

• Protein family: imidazoleglycerol-phosphate dehydratase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2AE8 (from Staphylococcus aureus subsp. aureus n315 mutant, 48% identity, 65% similarity)

• UniProt: O34683

• KEGG entry: [2]

• E.C. number: 4.2.1.19

Expression and regulation

• Operon: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI PubMed

• Expression browser: hisB PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)