PucR

• Description: transcriptional regulator of puc genes
  
  

Gene name	pucR
Synonyms	yunI
Essential	no
Product	transcriptional regulator of puc genes (PucR family)
Function	regulation of purine utilization
Gene expression levels in SubtiExpress: pucR
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Alternative nitrogen sources
MW, pI	60 kDa, 6.188
Gene length, protein length	1593 bp, 531 aa
Immediate neighbours	pucH, pucJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

• Locus tag: BSU32420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PucR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32138

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pucR-pucJ-pucK-pucL-pucM PubMed

• Expression browser: pucR PubMed

• Sigma factor:

• Regulation:
  • repressed by purine nucleotides (effector: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription auto-repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)