DnaB

• Description: initiation of chromosome replication/ membrane attachment protein, part of the replisome
  
  

Gene name	dnaB
Synonyms
Essential	yes PubMed
Product	initiation of chromosome replication/ membrane attachment protein
Function	DNA replication
Gene expression levels in SubtiExpress: dnaB
Interactions involving this protein in SubtInteract: DnaB
MW, pI	54 kDa, 5.278
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	dnaI, ytcG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU28990

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-PriA PubMed
  • DnaB-DnaI PubMed
  • DnaB-DnaC PubMed
  • DnaD-DnaB PubMed
  • DnaB-DnaX PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization:
  • Membrane-proximal (Spotty) PubMed
  • forms foci, close to oriC PubMed

Database entries

• Structure:

• UniProt: P07908

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dnaB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)