FolD

• Description: methylenetetrahydrofolate dehydrogenase (NADP)
  
  

Gene name	folD
Synonyms	yqiA
Essential	yes PubMed
Product	methylenetetrahydrofolate dehydrogenase (NADP)
Function	formylation of Met-tRNA(fMet)
Gene expression levels in SubtiExpress: folD
MW, pI	30 kDa, 5.6
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	yqiB, nusB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

• Locus tag: BSU24310

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + NADP⁺ = 5,10-methenyltetrahydrofolate + NADPH (according to Swiss-Prot)

• Protein family: tetrahydrofolate dehydrogenase/cyclohydrolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54382

• KEGG entry: [3]

• E.C. number: 1.5.1.5

Expression and regulation

• Operon: nusB-folD PubMed

• Expression browser: folD PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yann Duroc, Carmela Giglione, Thierry Meinnel
Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.
Antimicrob Agents Chemother: 2009, 53(4);1673-8
[PubMed:19171795] [WorldCat.org] [DOI] (I p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)