AnsA

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  • Description: L-asparaginase

Gene name ansA
Synonyms
Essential no
Product L-asparaginase
Function asparagine degradation
Gene expression levels in SubtiExpress: ansA
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 36 kDa, 4.455
Gene length, protein length 987 bp, 329 aa
Immediate neighbours ansB, ansR
Sequences Protein DNA DNA_with_flanks
Genetic context
AnsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsA expression.png



















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

  • Locus tag: BSU23580

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family: asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s): AnsZ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab
  • Expression vector:
  • lacZ fusion: pGP872 (in pAC5), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References