BshA

• Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis
  
  

Gene name	bshA
Synonyms	jojH, ypjH
Essential	no
Product	L-malic acid glycosyltransferase
Function	biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshA
MW, pI	41 kDa, 6.149
Gene length, protein length	1131 bp, 377 aa
Immediate neighbours	cca, bshB1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU22460

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed

• Protein family: NamA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3MBO (from B. anthracis) PubMed

• UniProt: P42982

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: bshA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: bshA::mls available in John_Helmann lab
  • GP88 (ypjH::pX2(cat)), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed

Heather Upton, Gerald L Newton, Melissa Gushiken, Kelly Lo, Dhiraj Holden, Robert C Fahey, Mamta Rawat
Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.
FEBS Lett: 2012, 586(7);1004-8
[PubMed:22569254] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333] [WorldCat.org] [DOI] (I p)