YngI

• Description: aceto-acetyl-CoA ligase, may be involved in surfactin production
  
  

Gene name	yngI
Synonyms
Essential	no
Product	aceto-acetyl-CoA ligase
Function	mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngI
MW, pI	61 kDa, 5.978
Gene length, protein length	1647 bp, 549 aa
Immediate neighbours	yngH, yngJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed

• Protein family:

• Paralogous protein(s): LcfA, LcfB, YhfT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31826

• KEGG entry: [3]

• E.C. number: 6.2.1.16

Expression and regulation

• Operon: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: