MtnB

• Description: methylthioribulose-1-phosphate dehydratase
  
  

Gene name	mtnB
Synonyms	ykrY
Essential	no
Product	methylthioribulose-1-phosphate dehydratase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnB
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	23 kDa, 5.686
Gene length, protein length	627 bp, 209 aa
Immediate neighbours	mtnX, mtnD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-methyl-5-thio-D-ribulose 1-phosphate = 5-(methylthio)-2,3-dioxopentyl phosphate + H₂O (according to Swiss-Prot)

• Protein family: MtnB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31668

• KEGG entry: [3]

• E.C. number: 4.2.1.109

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Expression browser: mtnB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: