YkfB

• Description: L-Ala-D/L-Glu epimerase
  
  

Gene name	ykfB
Synonyms
Essential	no
Product	L-Ala-D/L-Glu epimerase
Function	cell wall metabolism
Gene expression levels in SubtiExpress: ykfB
MW, pI	39 kDa, 5.905
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	ykfA, ykfC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU12980

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1TKK (complex with substrate), 1JPM

• UniProt: O34508

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykfB PubMed

• Sigma factor:

• Regulation: repressed by CodY PubMed

• Regulatory mechanism: CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tiit Lukk, Ayano Sakai, Chakrapani Kalyanaraman, Shoshana D Brown, Heidi J Imker, Ling Song, Alexander A Fedorov, Elena V Fedorov, Rafael Toro, Brandan Hillerich, Ronald Seidel, Yury Patskovsky, Matthew W Vetting, Satish K Nair, Patricia C Babbitt, Steven C Almo, John A Gerlt, Matthew P Jacobson
Homology models guide discovery of diverse enzyme specificities among dipeptide epimerases in the enolase superfamily.
Proc Natl Acad Sci U S A: 2012, 109(11);4122-7
[PubMed:22392983] [WorldCat.org] [DOI] (I p)

Vadim A Klenchin, Dawn M Schmidt, John A Gerlt, Ivan Rayment
Evolution of enzymatic activities in the enolase superfamily: structure of a substrate-liganded complex of the L-Ala-D/L-Glu epimerase from Bacillus subtilis.
Biochemistry: 2004, 43(32);10370-8
[PubMed:15301535] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

A M Gulick, D M Schmidt, J A Gerlt, I Rayment
Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2001, 40(51);15716-24
[PubMed:11747448] [WorldCat.org] [DOI] (P p)

D M Schmidt, B K Hubbard, J A Gerlt
Evolution of enzymatic activities in the enolase superfamily: functional assignment of unknown proteins in Bacillus subtilis and Escherichia coli as L-Ala-D/L-Glu epimerases.
Biochemistry: 2001, 40(51);15707-15
[PubMed:11747447] [WorldCat.org] [DOI] (P p)