Xis

Gene name	xis
Synonyms	ydzC, sacV
Essential	no
Product	excisionase
Function	excision of the conjugative transposon ICEBs1 from the trnS-leu2 locus
Gene expression levels in SubtiExpress: xis
MW, pI	7 kDa, 9.64
Gene length, protein length	192 bp, 64 aa
Immediate neighbours	immR, ydzL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

mobile genetic elements

This gene is a member of the following regulons

ImmR regulon

The gene

Basic information

• Locus tag: BSU04830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: excision of conjugative transposon PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31490

• KEGG entry: [2]

• E.C. number:

Additional information

Was reported to be involved in the regulation of the levansucrase gene (sacB)

Expression and regulation

• Operon:

• Expression browser: xis PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ImmR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Catherine A Lee, Jennifer M Auchtung, Rita E Monson, Alan D Grossman
Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 66(6);1356-69
[PubMed:18005101] [WorldCat.org] [DOI] (P p)

Jennifer M Auchtung, Catherine A Lee, Katherine L Garrison, Alan D Grossman
Identification and characterization of the immunity repressor (ImmR) that controls the mobile genetic element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 64(6);1515-28
[PubMed:17511812] [WorldCat.org] [DOI] (P p)