GsiB

• Description: general stress protein
  
  

Gene name	gsiB
Synonyms
Essential	no
Product	general stress protein
Function	unknown
Gene expression levels in SubtiExpress: gsiB
MW, pI	13 kDa, 5.145
Gene length, protein length	369 bp, 123 aa
Immediate neighbours	ydbA, ydbB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
[None Expression at a glance]   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigI regulon

The gene

Basic information

• Locus tag: BSU04400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P26907

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gsiB PubMed

• Expression browser: gsiB PubMed

• Sigma factor: SigB, SigI PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • 1A735 ( gsiB::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

B Jürgen, T Schweder, M Hecker
The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site.
Mol Gen Genet: 1998, 258(5);538-45
[PubMed:9669336] [WorldCat.org] [DOI] (P p)

B Maul, U Völker, S Riethdorf, S Engelmann, M Hecker
sigma B-dependent regulation of gsiB in response to multiple stimuli in Bacillus subtilis.
Mol Gen Genet: 1995, 248(1);114-20
[PubMed:7651322] [WorldCat.org] [DOI] (P p)

J P Mueller, G Bukusoglu, A L Sonenshein
Transcriptional regulation of Bacillus subtilis glucose starvation-inducible genes: control of gsiA by the ComP-ComA signal transduction system.
J Bacteriol: 1992, 174(13);4361-73
[PubMed:1378051] [WorldCat.org] [DOI] (P p)