YbgE

• Description: branched-chain amino acid aminotransferase
  
  

Gene name	ybgE
Synonyms
Essential	no
Product	branched-chain amino acid aminotransferase
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ybgE
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val, Coenzyme A
MW, pI	39 kDa, 4.952
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	ybgB, ybgF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU02390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s): YwaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31461

• KEGG entry: [3]

• E.C. number: 2.6.1.42

Expression and regulation

• Operon: ybgE PubMed

• Expression browser: ybgE PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • a ybgE mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab

• Expression vector:

• lacZ fusion: pGP248 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: