AlsD

• Description: acetolactate decarboxylase
  
  

Gene name	alsD
Synonyms
Essential	no
Product	acetolactate decarboxylase)
Function	overflow metabolism
Gene expression levels in SubtiExpress: alsD
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	28 kDa, 4.603
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	ywrO, alsS
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

AlsR regulon, Rex regulon, stringent response

The gene

Basic information

• Locus tag: BSU36000

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO₂ (according to Swiss-Prot)

• Protein family: alpha-acetolactate decarboxylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Ser-88 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q04777

• KEGG entry: [3]

• E.C. number: 4.1.1.5

Expression and regulation

• Operon: alsS-alsD PubMed

• Expression browser: alsD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by acetate (AlsR) PubMed
  • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
  • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

• Regulatory mechanism:
  • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
  • AlsR: transcription activation in the presence of acetate PubMed
  • Rex: transcription repression if the ratio NADH2/NAD is high PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP822, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed