BioF

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  • Description: 8-amino-7-oxononanoate synthase

Gene name bioF
Synonyms
Essential no
Product 8-amino-7-oxononanoate synthase
Function biosynthesis of biotin
Gene expression levels in SubtiExpress: bioF
Metabolic function and regulation of this protein in SubtiPathways:
Biotin
MW, pI 42 kDa, 6.285
Gene length, protein length 1167 bp, 389 aa
Immediate neighbours bioD, bioA
Sequences Protein DNA Advanced_DNA
Genetic context
BioF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BioF expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30220

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2 (according to Swiss-Prot)
  • Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications