BshB1

• Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis
  
  

Gene name	bshB1
Synonyms	jojG, ypjG
Essential	no
Product	N-acetylglucosamin-malate deacetylase
Function	biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshB1
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	24 kDa, 6.343
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	bshA, mgsA
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU22470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac

• Protein family:

• Paralogous protein(s): BshB2

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42981

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: bshB1 PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: bshB1 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
  • GP87 (ypjG::pX2(cat)), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333] [WorldCat.org] [DOI] (I p)