YeeK

• Description: spore coat protein
  
  

Gene name	yeeK
Synonyms
Essential	no
Product	spore coat protein
Function	unknown
Gene expression levels in SubtiExpress: yeeK
MW, pI	15 kDa, 6.852
Gene length, protein length	435 bp, 145 aa
Immediate neighbours	yeeI, yezE
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU06850

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • inner spore coat PubMed, assembles into the spore integument PubMed

Database entries

• Structure:

• UniProt: O31510

• KEGG entry: [2]

• E.C. number:

Additional information

CotE, SafA, and SpoVID are required for proper localization, clolocalizes with YabG PubMed

Expression and regulation

• Operon: yeeK PubMed

• Expression browser: yeeK PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expression starts 5 hrs after the onset of sporulation GerE PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S125 ( yeeK::erm), PubMed, available at BGSC
  • 1S125 ( yeeK::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Hiromu Takamatsu, Daisuke Imamura, Ritsuko Kuwana, Kazuhito Watabe
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.
J Bacteriol: 2009, 191(4);1220-9
[PubMed:19060142] [WorldCat.org] [DOI] (I p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)