CysS

• Description: cysteine-tRNA synthetase
  
  

Gene name	cysS
Synonyms	snpA
Essential	yes PubMed
Product	cysteine-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: cysS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Cys, Met & Sulfate assimilation
MW, pI	53 kDa, 5.07
Gene length, protein length	1398 bp, 466 aa
Immediate neighbours	cysE, mrnC
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU00940

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-270 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1U0B (from Escherichia coli, 42% identity, 56% similarity) PubMed

• UniProt: Q06752

• KEGG entry: [3]

• E.C. number: 6.1.1.16

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Expression browser: cysS PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)