KtrA

• Description: high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
  
  

Gene name	ktrA
Synonyms	yuaA
Essential	no
Product	high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
Function	potassium uptake
Gene expression levels in SubtiExpress: ktrA
Interactions involving this protein in SubtInteract: KtrA
Metabolic function and regulation of this protein in SubtiPathways: Metal ion homeostasis, Stress
MW, pI	24 kDa, 5.981
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	bslA, ktrB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

YdaO riboswitch

The gene

Basic information

• Locus tag: BSU31090

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): KtrC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • KtrA-KtrB PubMed, composed of an octameric KtrA ring and two KtrB dimers PubMed

• Localization: peripheral membrane protein PubMed

Database entries

• Structure:
  • 1LSU (complex with NADH)
  • 2HMW ( complex with ATP)
  • 4J7C (the KtrA-KtrB complex) PubMed

• UniProt: O32080

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ktrA-ktrB PubMed

• Expression browser: ktrA PubMed

• Sigma factor:

• Regulation:
  • induced if the energy charge is low (ydaO riboswitch) PubMed

• Regulatory mechanism:
  • expression is controlled via termination antitermination by the ydaO riboswitch PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A954 ( ktrA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Ricardo S Vieira-Pires, Andras Szollosi, João H Morais-Cabral
The structure of the KtrAB potassium transporter.
Nature: 2013, 496(7445);323-8
[PubMed:23598340] [WorldCat.org] [DOI] (I p)

Peter Y Watson, Martha J Fedor
The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.
Nat Chem Biol: 2012, 8(12);963-5
[PubMed:23086297] [WorldCat.org] [DOI] (I p)

Kirsten F Block, Ming C Hammond, Ronald R Breaker
Evidence for widespread gene control function by the ydaO riboswitch candidate.
J Bacteriol: 2010, 192(15);3983-9
[PubMed:20511502] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)

Gudrun Holtmann, Evert P Bakker, Nobuyuki Uozumi, Erhard Bremer
KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity.
J Bacteriol: 2003, 185(4);1289-98
[PubMed:12562800] [WorldCat.org] [DOI] (P p)