BacG

• Description: short chain reductase involved in bacilysin synthesis
  
  

Gene name	bacG
Synonyms	ipa-86r, ywfH
Essential	no
Product	short chain reductase
Function	biosynthesis of the antibiotic bacilysin
Gene expression levels in SubtiExpress: bacG
MW, pI	27 kDa, 6.596
Gene length, protein length	777 bp, 259 aa
Immediate neighbours	hemQ, bacF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU37680

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate PubMed
  • stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP PubMed

• Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): NADPH PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3U49 PubMed

• UniProt: P39644

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bacG PubMed

• Expression browser: bacG PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Malligarjunan Rajavel, Kumar Perinbam, B Gopal
Structural insights into the role of Bacillus subtilis YwfH (BacG) in tetrahydrotyrosine synthesis.
Acta Crystallogr D Biol Crystallogr: 2013, 69(Pt 3);324-32
[PubMed:23519407] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839] [WorldCat.org] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)