YrvM

• Description: similar to E. coli enzyme that catalyzes ATP-dependent dehydration of N6-threonylcarbamoyladenosine (t6A) to form cyclic N6-threonylcarbamoyladenosine (ct6A)
  
  

Gene name	yrvM
Synonyms
Essential	no
Product	N6-threonylcarbamoyladenosine cyclase
Function	tRNA modification
Gene expression levels in SubtiExpress: yrvM
MW, pI	28 kDa, 8.187
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	yrvN, bsrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU27540

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • catalyzes ATP-dependent dehydration of N6-threonylcarbamoyladenosine (t6A) to form cyclic N6-threonylcarbamoyladenosine (ct6A) (ct6A recognizes the first adenine base of ANN codon at the ribosomal A site) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32037

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yrvM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kenjyo Miyauchi, Satoshi Kimura, Tsutomu Suzuki
A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification.
Nat Chem Biol: 2013, 9(2);105-11
[PubMed:23242255] [WorldCat.org] [DOI] (I p)