YngJ

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  • Description: 3-methylbutanoyl-CoA dehydrogenase

Gene name yngJ
Synonyms
Essential no
Product 3-methylbutanoyl-CoA dehydrogenase
Function mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngJ
MW, pI 40 kDa, 5.794
Gene length, protein length 1140 bp, 380 aa
Immediate neighbours yngI, ynzE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YngJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YngJ expression.png















Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU18260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 3-methylbutanoyl-CoA --> 3-methylbut-2-enoyl-CoA PubMed
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3OWA (from B. anthracis, 37% identity, 54% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)