YlaM

• Description: glutaminase, high affinity for glutamine
  
  

Gene name	ylaM
Synonyms
Essential	no
Product	glutaminase
Function	glutamine degradation
Gene expression levels in SubtiExpress: ylaM
MW, pI	33 kDa, 5.676
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	ylaL, ylaN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

The gene

Basic information

• Locus tag: BSU14830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamine + H₂O = L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: glutaminase family (according to Swiss-Prot)

• Paralogous protein(s): GlsA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BRM (the structure of GlsA) PubMed

• UniProt: O07637

• KEGG entry: [2]

• E.C. number: 3.5.1.2

Expression and regulation

• Operon:

• Expression browser: ylaM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799] [WorldCat.org] [DOI] (I p)