MtlF

• Description: phosphotransferase system (PTS) mannitol-specific enzyme IIA component
  
  

Gene name	mtlF
Synonyms
Essential	no
Product	phosphotransferase system(PTS) mannitol-specific enzyme IIA component
Function	uptake of mannitol
Gene expression levels in SubtiExpress: mtlF
Interactions involving this protein in SubtInteract: MtlF
MW, pI	16 kDa, 4.496
Gene length, protein length	429 bp, 143 aa
Immediate neighbours	mtlA, mtlD
Gene sequence (+200bp)	Protein sequence
Genetic context File:MtlF context.gif

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

• Locus tag: BSU03982

Database entries

• DBTBS entry: no entry

• SubtiList entry: no entry

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PtsH-MtlF PubMed
  • MtlA-MtlF PubMed
  • MtlF-MtlR PubMed

• Localization:

Database entries

• Structure:

• UniProt: C0H3V2

• KEGG entry:

• E.C. number:

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Sigma factor:

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094] [WorldCat.org] [DOI] (I p)

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)