CcpN

• Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
  
  

Gene name	ccpN
Synonyms	yqzB
Essential	no
Product	transcriptional regulator
Function	repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1
Gene expression levels in SubtiExpress: ccpN
Interactions involving this protein in SubtInteract: CcpN
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism
MW, pI	23.4 kDa, 7.22
Gene length, protein length	636 bp, 212 amino acids
Immediate neighbours	yqfL, glyS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

The CcpN regulon:

• gapB-speD, pckA, Sr1

The gene

Basic information

• Locus tag: BSU25250

Phenotypes of a mutant

Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • YqfL (negative effector of CcpN activity) PubMed
  • ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed

• Interactions:
  • active as dimer (according to PubMed)
  • CcpN-RpoA PubMed

• Localization:

Database entries

• Structure:

• UniProt: O34994

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ccpN-yqfL PubMed

• Expression browser: ccpN PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Additional information:
  • the intracellular concentration of CcpN is about 4 myM (according to PubMed).

Biological materials

• Mutant:
  • DB104 ccpN::cat, available in Sabine Brantl's lab
  • GP1128 ccpN::cat, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody: available in Sabine Brantl's lab

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage

Uwe Sauer, ETH Zürich, Switzerland homepage

References

Reviews

Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793] [WorldCat.org] [DOI] (I p)

Original Publications

Matthew L Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A Royer
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.
Proc Natl Acad Sci U S A: 2012, 109(1);155-60
[PubMed:22190493] [WorldCat.org] [DOI] (I p)

Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol Lett: 2009, 299(2);223-31
[PubMed:19732150] [WorldCat.org] [DOI] (I p)

Andreas Licht, Sabine Brantl
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
J Biol Chem: 2009, 284(44);30032-8
[PubMed:19726675] [WorldCat.org] [DOI] (I p)

Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J Bacteriol: 2008, 190(18);6178-87
[PubMed:18586936] [WorldCat.org] [DOI] (I p)

Andreas Licht, Ralph Golbik, Sabine Brantl
Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis.
J Mol Biol: 2008, 380(1);17-30
[PubMed:18511073] [WorldCat.org] [DOI] (I p)

Andreas Licht, Sabine Brantl
Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding.
J Mol Biol: 2006, 364(3);434-48
[PubMed:17011578] [WorldCat.org] [DOI] (P p)

Andreas Licht, Sven Preis, Sabine Brantl
Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis.
Mol Microbiol: 2005, 58(1);189-206
[PubMed:16164558] [WorldCat.org] [DOI] (P p)

Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552] [WorldCat.org] [DOI] (P p)