CcpN
- Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
Gene name | ccpN |
Synonyms | yqzB |
Essential | no |
Product | transcriptional regulator |
Function | repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1 |
Gene expression levels in SubtiExpress: ccpN | |
Interactions involving this protein in SubtInteract: CcpN | |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism | |
MW, pI | 23.4 kDa, 7.22 |
Gene length, protein length | 636 bp, 212 amino acids |
Immediate neighbours | yqfL, glyS |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, transcription factors and their control, regulators of core metabolism
This gene is a member of the following regulons
The CcpN regulon:
The gene
Basic information
- Locus tag: BSU25250
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34994
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation: constitutively expressed PubMed
- Additional information:
- the intracellular concentration of CcpN is about 4 myM (according to PubMed).
Biological materials
- Mutant:
- DB104 ccpN::cat, available in Sabine Brantl lab
- GP1128 ccpN::cat, available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody: available in Sabine Brantl lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Uwe Sauer, ETH Zürich, Switzerland homepage
Your additional remarks
References
Reviews
Original Publications
Matthew L Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A Royer
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.
Proc Natl Acad Sci U S A: 2012, 109(1);155-60
[PubMed:22190493]
[WorldCat.org]
[DOI]
(I p)
Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol Lett: 2009, 299(2);223-31
[PubMed:19732150]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
J Biol Chem: 2009, 284(44);30032-8
[PubMed:19726675]
[WorldCat.org]
[DOI]
(I p)
Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J Bacteriol: 2008, 190(18);6178-87
[PubMed:18586936]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Ralph Golbik, Sabine Brantl
Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis.
J Mol Biol: 2008, 380(1);17-30
[PubMed:18511073]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding.
J Mol Biol: 2006, 364(3);434-48
[PubMed:17011578]
[WorldCat.org]
[DOI]
(P p)
Andreas Licht, Sven Preis, Sabine Brantl
Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis.
Mol Microbiol: 2005, 58(1);189-206
[PubMed:16164558]
[WorldCat.org]
[DOI]
(P p)
Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552]
[WorldCat.org]
[DOI]
(P p)