ManA

• Description: mannose-6-phosphate isomerase, required for proper cell wall synthesis
  
  

Gene name	manA
Synonyms	yjdE
Essential	no
Product	mannose-6-phosphate isomerase
Function	mannose utilization
Gene expression levels in SubtiExpress: manA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	35 kDa, 5.279
Gene length, protein length	945 bp, 315 aa
Immediate neighbours	manP, yjdF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

ManR regulon

The gene

Basic information

• Locus tag: BSU12020

Phenotypes of a mutant

• cells lose the characteristic rod shape typical of wild type B. subtilis cells and instead appear as elongated spheres, which are significantly larger than normal PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31646

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manP-manA-yjdF PubMed

• Expression browser: manA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Maya Elbaz, Sigal Ben-Yehuda
The metabolic enzyme ManA reveals a link between cell wall integrity and chromosome morphology.
PLoS Genet: 2010, 6(9);e1001119
[PubMed:20862359] [WorldCat.org] [DOI] (I e)

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

M S Turner, J D Helmann
Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis.
J Bacteriol: 2000, 182(18);5202-10
[PubMed:10960106] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)