NasA

• Description: nitrate transporter
  
  

Gene name	nasA
Synonyms
Essential	no
Product	nitrate transporter
Function	nitrate uptake
Gene expression levels in SubtiExpress: nasA
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	45 kDa, 10.494
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	nasB, yciA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU03330

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s): NarK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P42432

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: nasA PubMed

• Sigma factor:

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A972 ( nasA::phleo), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

K Ogawa, E Akagawa, K Yamane, Z W Sun, M LaCelle, P Zuber, M M Nakano
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(5);1409-13
[PubMed:7868621] [WorldCat.org] [DOI] (P p)

M M Nakano, F Yang, P Hardin, P Zuber
Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(3);573-9
[PubMed:7836289] [WorldCat.org] [DOI] (P p)