SpoIISA

• Description: lethal when synthesized during vegetative growth in the absence of SpoIISB
  
  

Gene name	spoIISA
Synonyms	ykaC
Essential	no
Product	toxin
Function	programmed cell death
Gene expression levels in SubtiExpress: spoIISA
Interactions involving this protein in SubtInteract: SpoIISA
MW, pI	28 kDa, 4.683
Gene length, protein length	744 bp, 248 aa
Immediate neighbours	spoIISB, pit
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU12830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: three putative membrane-spanning segments and a cytoplasmic domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIISA-SpoIISB PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure: 3O6Q (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed

• UniProt: O34853

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: spoIISA-spoIISB PubMed

• Expression browser: spoIISA PubMed

• Sigma factor:

• Regulation:
• levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S123 ( spoIISA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)

Additional Publications: PubMed