YkgB

• Description: 6-phosphogluconolactonase, general stress protein
  
  

Gene name	ykgB
Synonyms
Essential	no
Product	6-phosphogluconolactonase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ykgB
MW, pI	38 kDa, 5.154
Gene length, protein length	1047 bp, 349 aa
Immediate neighbours	ykfD, ykgA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), carbon core metabolism

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU13010

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 6-phosphogluconolactone --> 6-phosphogluconate (pentose phosphate pathway) PubMed

• Protein family: cycloisomerase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34499

• KEGG entry: [3]

• E.C. number: 3.1.1.31

Expression and regulation

• Operon: ykgA-ykgB PubMed

• Expression browser: ykgB PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1402 (spc, based on pGP1331), available in the Stülke lab

References

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Lynn C Thomason, Donald L Court, Atin R Datta, Rita Khanna, Judah L Rosner
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.
J Bacteriol: 2004, 186(24);8248-53
[PubMed:15576773] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)