PpaC

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  • Description: inorganic pyrophosphatase

Gene name ppaC
Synonyms yybQ
Essential yes PubMed
Product inorganic pyrophosphatase
Function recovery of phosphate ions from pyrophosphate
Gene expression levels in SubtiExpress: ppaC
MW, pI 33 kDa, 4.513
Gene length, protein length 927 bp, 309 aa
Immediate neighbours hypR, yybP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PpaC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

phosphate metabolism, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Diphosphate + H2O = 2 phosphate (according to Swiss-Prot)
  • Protein family: PPase class C family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • S-cysteinylation after diamide stress (C158) PubMed
    • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: ppaC (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References