MetE

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  • Description: methionine synthase

Gene name metE
Synonyms metC
Essential no
Product methionine synthase
Function biosynthesis of methionine
Gene expression levels in SubtiExpress: metE
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 86 kDa, 4.839
Gene length, protein length 2286 bp, 762 aa
Immediate neighbours guaD, ispA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MetE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetE expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

This gene is a member of the following regulons

S-box

The gene

Basic information

  • Locus tag: BSU13180

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine (according to Swiss-Prot)
  • Protein family: vitamin-B12 independent methionine synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
    • Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
    • MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed


  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1T7L (from Thermotoga maritima, 44% identity, 61% similarity) PubMed
  • KEGG entry: [2]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Operon: metE
  • Sigma factor:
  • Regulation:
    • repressed by casamino acids PubMed
    • repressed by methionine (about 1000-fold)(S-box) PubMed
  • Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed