Rnz

• Description: RNase Z
  
  

Gene name	rnz
Synonyms	yqjK
Essential	yes PubMed
Product	endoribonuclease Z
Function	processing of CCA-less tRNA precursors
Gene expression levels in SubtiExpress: rnz
MW, pI	33 kDa, 5.805
Gene length, protein length	921 bp, 307 aa
Immediate neighbours	yqjL, zwf
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, translation, essential genes

The gene

Basic information

• Locus tag: BSU23840

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs (according to Swiss-Prot)

• Protein family: RNase Z family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2FK6 (in complex with tRNA-Thr) PubMed, 1Y44

• UniProt: P54548

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnz (according to DBTBS)

• Expression browser: rnz PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

References

Original Publications

Olivier Pellegrini, Inés Li de la Sierra-Gallay, Jérémie Piton, Laetitia Gilet, Ciarán Condon
Activation of tRNA maturation by downstream uracil residues in B. subtilis.
Structure: 2012, 20(10);1769-77
[PubMed:22940585] [WorldCat.org] [DOI] (I p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)

Inés Li de la Sierra-Gallay, Nathalie Mathy, Olivier Pellegrini, Ciarán Condon
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA.
Nat Struct Mol Biol: 2006, 13(4);376-7
[PubMed:16518398] [WorldCat.org] [DOI] (P p)

Inés Li de la Sierra-Gallay, Olivier Pellegrini, Ciarán Condon
Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z.
Nature: 2005, 433(7026);657-61
[PubMed:15654328] [WorldCat.org] [DOI] (I p)

Olivier Pellegrini, Jamel Nezzar, Anita Marchfelder, Harald Putzer, Ciarán Condon
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis.
EMBO J: 2003, 22(17);4534-43
[PubMed:12941704] [WorldCat.org] [DOI] (P p)