YxeI

• Description: penicillin acylase
  
  

Gene name	yxeI
Synonyms
Essential	no
Product	penicillin acylase
Function	resistance to penicillin
Gene expression levels in SubtiExpress: yxeI
MW, pI	37 kDa, 5.276
Gene length, protein length	984 bp, 328 aa
Immediate neighbours	yxeJ, yxeH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Resistance against toxins/ antibiotics

The gene

Basic information

• Locus tag: BSU39540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acylation of penicillin V PubMed

• Protein family: peptidase C59 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • homotetramer PubMed

• Localization:

Database entries

• Structure: 2OQC

• UniProt: P54948

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yxeI-yxeJ PubMed

• Expression browser: yxeI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Priya Rathinaswamy, Sushama M Gaikwad, C G Suresh, Asmita A Prabhune, James A Brannigan, Guy G Dodson, Archana V Pundle
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int J Biol Macromol: 2012, 50(1);25-30
[PubMed:21978958] [WorldCat.org] [DOI] (I p)

Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)