TuaA/1

• Description: lipid carrier sugar transferase, disrupted pseudogene in B. subtilis 168
  
  

Gene name	tuaA
Synonyms	yvhA
Essential	no
Product	lipid carrier sugar transferase
Function	biosynthesis of teichuronic acid
Gene expression levels in SubtiExpress: tuaA/1
MW, pI	- , -
Gene length, protein length	150 bp, -
Immediate neighbours	tuaB, tuaA/2
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, membrane proteins, pseudogenes

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU35609

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetyl-D-galactosamine + undecaprenyl phosphate = UMP + N-acetyl-alpha-D-galactosaminyldiphospho-undecaprenol (according to Swiss-Prot)

• Protein family: bacterial sugar transferase family (according to Swiss-Prot)

• Paralogous protein(s): EpsL

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O32274

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tuaA-tuaB-tuaC-tuaD-tuaE-tuaF-tuaG-tuaH PubMed

• Expression browser: tuaA/1 PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maryam Lahooti, Colin R Harwood
Transcriptional analysis of the Bacillus subtilis teichuronic acid operon.
Microbiology (Reading): 1999, 145 ( Pt 12);3409-3417
[PubMed:10627039] [WorldCat.org] [DOI] (P p)

B Soldo, V Lazarevic, M Pagni, D Karamata
Teichuronic acid operon of Bacillus subtilis 168.
Mol Microbiol: 1999, 31(3);795-805
[PubMed:10048024] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

Wei Liu, F Marion Hulett
Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho-regulon promoters establishes a Bacillus subtilis Pho core binding site.
Microbiology (Reading): 1998, 144 ( Pt 5);1443-1450
[PubMed:9611818] [WorldCat.org] [DOI] (P p)