DnaK

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  • Description: class I heat-shock protein (molecular chaperone)

Gene name dnaK
Synonyms
Essential no
Product class I heat-shock protein (molecular chaperone)
Function protein quality control
Gene expression levels in SubtiExpress: dnaK
Interactions involving this protein in SubtInteract: DnaK
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 65 kDa, 4.571
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours surC, grpE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DnaK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaK expression.png




























Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU25470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: heat shock protein 70 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • membrane-proximal (Spotty) PubMed
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

Additional publications: PubMed