Pgk
- Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme
Gene name | pgk |
Synonyms | |
Essential | yes |
Product | phosphoglycerate kinase |
Function | enzyme in glycolysis/ gluconeogenesis |
MW, pI | 42,0 kDa, 4.77 |
Gene length, protein length | 1182 bp, 394 amino acids |
Immediate neighbours | gapA, tpi |
Gene sequence (+200bp) | Protein sequence |
Genetic context |
Contents
The gene
Basic information
- Coordinates: 3479231 - 3480412
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate
- Protein family: phosphoglycerate kinase family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- nucleotide binding domain (ATP) (350–353)
- 2x substrate binding domain (21–23), (59–62)
- Modification: phosphorylation PubMed,
- Cofactor(s):
- Effectors of protein activity:
- Interactions: Pgk-GapA
- Localization: Cytoplasm PubMed
Database entries
- Structure: Geobacillus stearothermophilus NCBI
- Swiss prot entry: [3]
- KEGG entry: [4]
- E.C. number: [5]
Additional information
Expression and regulation
- Sigma factor: SigA
- Regulation:
cggR: neg. regulated by CggR PubMed, induced by sugar
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed
- Jannière, L., Canceill, D., Suski, C., Kanga, S., Dalmais, B., Lestini, R., Monnier, A. F., Chapuis, J., Bolotin, A., Titok, M., Le Chatelier, E., and Ehrlich, S. D. (2007) Genetic evidence for a link between glycolysis and DNA replication. PLoS ONE 2, e447. PubMed