Ldt

• Description: L,D-transpeptidase involved in cell wall synthesis
  
  

Gene name	ldt
Synonyms	ykuD
Essential	no
Product	L,D-transpeptidase
Function	cell wall biosynthesis
MW, pI	17 kDa, 10.252
Gene length, protein length	492 bp, 164 aa
Immediate neighbours	ykuC, ykuE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, sporulation proteins

This gene is a member of the following regulons

CcpA regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU14040

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LysM repeat (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • spore wall (according to Swiss-Prot)

Database entries

• Structure: 1Y7M

• UniProt: O34816

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ldt PubMed

• Expression browser: ldt PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • repressed by glucose (3.3-fold) (CcpA) PubMed
  • expressed during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism: CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Lauriane Lecoq, Catherine Bougault, Jean-Emmanuel Hugonnet, Carole Veckerlé, Ombeline Pessey, Michel Arthur, Jean-Pierre Simorre
Dynamics induced by β-lactam antibiotics in the active site of Bacillus subtilis L,D-transpeptidase.
Structure: 2012, 20(5);850-61
[PubMed:22579252] [WorldCat.org] [DOI] (I p)

Sophie Magnet, Ana Arbeloa, Jean-Luc Mainardi, Jean-Emmanuel Hugonnet, Martine Fourgeaud, Lionel Dubost, Arul Marie, Vanessa Delfosse, Claudine Mayer, Louis B Rice, Michel Arthur
Specificity of L,D-transpeptidases from gram-positive bacteria producing different peptidoglycan chemotypes.
J Biol Chem: 2007, 282(18);13151-9
[PubMed:17311917] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

T Kodama, H Takamatsu, K Asai, N Ogasawara, Y Sadaie, K Watabe
Synthesis and characterization of the spore proteins of Bacillus subtilis YdhD, YkuD, and YkvP, which carry a motif conserved among cell wall binding proteins.
J Biochem: 2000, 128(4);655-63
[PubMed:11011148] [WorldCat.org] [DOI] (P p)