BioF

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  • Description: 8-amino-7-oxononanoate synthase

Gene name bioF
Synonyms
Essential no
Product 8-amino-7-oxononanoate synthase)
Function biosynthesis of biotin
Metabolic function and regulation of this protein in SubtiPathways:
Biotin
MW, pI 42 kDa, 6.285
Gene length, protein length 1167 bp, 389 aa
Immediate neighbours bioD, bioA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BioF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BioF expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30220

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2 (according to Swiss-Prot)
  • Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications