YwaA

• Description: branched-chain amino acid aminotransferase
  
  

Gene name	ywaA
Synonyms	ipa-0r
Essential	no
Product	branched-chain amino acid aminotransferase
Function	biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val
MW, pI	40 kDa, 4.952
Gene length, protein length	1089 bp, 363 aa
Immediate neighbours	dltE, licH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU38550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s): YbgE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: S-cysteinylation after diamide stress (C104) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HT5 (from Mycobacterium tuberculosis, 42% identity, 58% similarity) PubMed

• UniProt: P39576

• KEGG entry: [3]

• E.C. number: 2.6.1.42

Expression and regulation

• Operon: ywaA PubMed

• Expression browser: ywaA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • a ywaA::spc mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Falko Hochgräfe, Jörg Mostertz, Dierk-Christoph Pöther, Dörte Becher, John D Helmann, Michael Hecker
S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress.
J Biol Chem: 2007, 282(36);25981-5
[PubMed:17611193] [WorldCat.org] [DOI] (P p)

Bradley J Berger, Shane English, Gene Chan, Marvin H Knodel
Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.
J Bacteriol: 2003, 185(8);2418-31
[PubMed:12670965] [WorldCat.org] [DOI] (P p)