AhrC
Revision as of 22:40, 13 January 2009 by 82.82.75.193 (talk)
- Description: AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism.
Gene name | ahrC |
Synonyms | argR |
Essential | no |
Product | transcriptional regulator |
Function | transcriptional regulator of arginine metabolic genes |
MW, pI | 16,7 kDa, 5.52 |
Gene length, protein length | 447 bp, 149 amino acids |
Immediate neighbours | yqxC, recN |
Gene sequence (+200bp) | Protein sequence |
Genetic context |
Contents
The gene
Basic information
- Coordinates: 2521558 - 2522004
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcriptional activator/ repressor of genes involved in arginine metabolism
- Protein family: ArgR family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): L-arginine is the co-factor required for transcription repression/ activation
- Effectors of protein activity:
- Interactions:
- Localization: Cytoplasm
Database entries
- Swiss prot entry: [3]
- KEGG entry: [4]
Additional information
Expression and regulation
- Operon: ahrC-recN
- Sigma factor:
- Regulation: by small RNA sr1
- Additional information:
Biological materials
Labs working on this gene/protein
Simon Phillips, Leeds University, UK Homepage
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
- Van Hoy BE et al. (1990) Characterization of the spoIVB and recN loci of Bacillus subtilis J Bacteriol. 172: 1306-1311. PubMed
- Heidrich et al. (2006) The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism. Mol. Microbiol. 62: 520-536. PubMed
- Heidrich et al. (2007) In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA. Nucl. Acids Res. 35: 4331-4346. PubMed
- Garnett et al. (2008) Structure and function of the arginine repressor-operator complex from Bacillus subtilis. J. Mol. Biol. 379: 284-298. PubMed
- Garnett et al. (2007) Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine. Acta Cryst. Sect. F. 63: 918-921. PubMed
- Garnett et al. (2007) A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis. Acta Cryst. Sect. F. 63: 914-917. PubMed
- Dennis et al. (2002) The structure of AhrC, the arginine repressor/activator protein from Bacillus subtilis. Acta Cryst. Sect. D. 58:421-430. PubMed
- Makarova et al. (2001) Conservation of the binding site for the arginine repressor in all bacterial lineages. Genome Biol. 2: RESEARCH0013. PubMed
- Miller et al. (1997) Operator interactions by the Bacillus subtilis arginine repressor/ activator, AhrC: novel positioning and DNA-mediated assembly of a transcriptional activator at catabolic sites. Mol. Microbiol. 26: 37-48. PubMed
- Klingel et al. (1995) A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/ activator of arginine metabolism. Mol. Gen. Genet. 248:329-340. PubMed
- Czaplewski et al. (1992) Purification and initial characterization of AhrC: the regulator of arginine metabolism genes in Bacillus subtilis. Mol. Microbiol. 6:267-275. PubMed
- North et al. (1989) Nucleotide sequence of a Bacillus subtilis arginine regulatory gene and homology of its product to the Escherichia coli arginine repressor. Gene 80:29-38. 2507400 PubMed