RacE

• Description: glutamate racemase
  
  

Gene name	racE
Synonyms	glr
Essential	yes PubMed
Product	glutamate racemase
Function	peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	29 kDa, 4.858
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	gerM, ysmB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

The gene

Basic information

• Locus tag: BSU28390

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamate = D-glutamate (according to Swiss-Prot)

• Protein family: aspartate/glutamate racemases family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1ZUW (with D-glutamate)

• UniProt: P94556

• KEGG entry: [2]

• E.C. number: 5.1.1.3

Expression and regulation

• Operon:

• Expression browser: racE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Ashley Spies, Joseph G Reese, Dylan Dodd, Katherine L Pankow, Steven R Blanke, Jerome Baudry
Determinants of catalytic power and ligand binding in glutamate racemase.
J Am Chem Soc: 2009, 131(14);5274-84
[PubMed:19309142] [WorldCat.org] [DOI] (I p)

Eduard Puig, Edgar Mixcoha, Mireia Garcia-Viloca, Angels González-Lafont, José M Lluch
How the substrate D-glutamate drives the catalytic action of Bacillus subtilis glutamate racemase.
J Am Chem Soc: 2009, 131(10);3509-21
[PubMed:19227983] [WorldCat.org] [DOI] (I p)

Sergey N Ruzheinikov, Makie A Taal, Svetlana E Sedelnikova, Patrick J Baker, David W Rice
Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery.
Structure: 2005, 13(11);1707-13
[PubMed:16271894] [WorldCat.org] [DOI] (P p)

Keitarou Kimura, Lam-Son Phan Tran, Yoshifumi Itoh
Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis.
Microbiology (Reading): 2004, 150(Pt 9);2911-2920
[PubMed:15347750] [WorldCat.org] [DOI] (P p)