SsbA

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  • Description: single-strand DNA-binding protein, part of the replisome

Gene name ssbA
Synonyms ssb
Essential yes PubMed
Product single-strand DNA-binding protein
Function DNA replication, DNA repair/ recombination
Interactions involving this protein in SubtInteract: SsbA
MW, pI 18 kDa, 4.822
Gene length, protein length 516 bp, 172 aa
Immediate neighbours rpsR, rpsF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ssb context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA replication, DNA repair/ recombination, essential genes

This gene is a member of the following regulons

ComK regulon, stringent response

The gene

Basic information

  • Locus tag: BSU40900

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • SsbA inhibits ssDNA phosphorylase activity of PnpA PubMed
    • SsbA co-assembles onto SsbB-coated ssDNA PubMed
  • Protein family:
  • Paralogous protein(s): SsbB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Tyr-82 by PtkA, dephosphorylated by PtpZ PubMed
  • Cofactor(s):
  • Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed

Database entries

  • Structure: 3VDY 3VDY (SsbB in complex with ssDNA, 63% identity, 87% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
    • ComK: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed